|  Help  |  About  |  Contact Us

Allele : Gt(ROSA)26Sor<tm1Acoh> gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Adam Cohen
Primary Identifier  MGI:5771697 Allele Type  Targeted
Attribute String  Conditional ready, Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  (129S6/SvEvTac x C57BL/6NCrl)F1
Is Recombinase  false Is Wild Type  false
molecularNote  A cassette containing pCAG-FRT-loxp-Stop codons-3x SV40 polyA-loxP-Optopatch2-WPRE (woodchuck hepatitis post-transcriptional regulatory element)-bGHpA (bovine growth hormone polyadenylation signal)-AttB-pPGK-FRT-neo-pA-AttP was introduced to intron 1. Optopatch2 is bicistronic construct that coexpresses QuasAr2-dark mOrange2 (an enhanced archaerhodopsin-3 (Arch)-based voltage indicator) and CheRiff-EGFP (a sensitive blue-shifted optogenetic actuator) separated by the porcine teschovirus-1 (P2A) peptide. This peptide causes a ribosomal skip, leading to near-balanced co-expression of the QuasAr2-dark mOrange2 and CheRiff-EGFP upon cre-mediated excision of the floxed Stop cassette.
  • mutations:
  • Insertion
  • synonyms:
  • Ai130,
  • Floxopatch,
  • Ai130,
  • Floxopatch
Paste the following link
Quick Links:
SummaryGenomePhenotypeOther
 
Quick Links:
SummaryGenomePhenotypeOther
 
Lists
This Allele isn't in any lists. Upload a list.

1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

0 Driven By

4 Publication categories

Trail: Allele




MouseMine is a collaboration between MGI at The Jackson Laboratory and the InterMine project at the Cambridge Systems Biology Centre. MouseMine is funded through a grant from the NIH/NHGRI.The Jackson Laboratory makes no representation about the suitability or accuracy of this software or data for any purpose, and makes no warranties, either express or implied, including merchantability and fitness for a particular purpose or that the use of this software or data will not infringe any third party patents, copyrights, trademarks, or other rights. the software and data are provided "as is". This software and data are provided to enhance knowledge and encourage progress in the scientific community and are to be used only for research and educational purposes. Any reproduction or use for commercial purpose is prohibited without the prior express written permission of the Jackson Laboratory.

Copyright © 2017 by The Jackson Laboratory. All Rights Reserved

© 2002 - 2017 Department of Genetics, University of Cambridge, Downing Street,
Cambridge CB2 3EH, United Kingdom