| First Author | Kepser LJ | Year | 2019 |
| Journal | Proc Natl Acad Sci U S A | Volume | 116 |
| Issue | 17 | Pages | 8397-8402 |
| PubMed ID | 30962377 | Mgi Jnum | J:277814 |
| Mgi Id | MGI:6295710 | Doi | 10.1073/pnas.1813351116 |
| Citation | Kepser LJ, et al. (2019) CAP2 deficiency delays myofibril actin cytoskeleton differentiation and disturbs skeletal muscle architecture and function. Proc Natl Acad Sci U S A 116(17):8397-8402 |
| abstractText | Actin filaments (F-actin) are key components of sarcomeres, the basic contractile units of skeletal muscle myofibrils. A crucial step during myofibril differentiation is the sequential exchange of alpha-actin isoforms from smooth muscle (alpha-SMA) and cardiac (alpha-CAA) to skeletal muscle alpha-actin (alpha-SKA) that, in mice, occurs during early postnatal life. This "alpha-actin switch" requires the coordinated activity of actin regulators because it is vital that sarcomere structure and function are maintained during differentiation. The molecular machinery that controls the alpha-actin switch, however, remains enigmatic. Cyclase-associated proteins (CAP) are a family of actin regulators with largely unknown physiological functions. We here report a function for CAP2 in regulating the alpha-actin exchange during myofibril differentiation. This alpha-actin switch was delayed in systemic CAP2 mutant mice, and myofibrils remained in an undifferentiated stage at the onset of the often excessive voluntary movements in postnatal mice. The delay in the alpha-actin switch coincided with the onset of motor function deficits and histopathological changes including a high frequency of type IIB ring fibers. Our data suggest that subtle disturbances of postnatal F-actin remodeling are sufficient for predisposing muscle fibers to form ring fibers. Cofilin2, a putative CAP2 interaction partner, has been recently implicated in myofibril actin cytoskeleton differentiation, and the myopathies in cofilin2 and CAP2 mutant mice showed striking similarities. We therefore propose a model in which CAP2 and cofilin2 cooperate in actin regulation during myofibril differentiation. |
