| Primary Identifier | MGI:5788506 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Ncbp1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele from project Ncbp1-7919J-M3862 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences AATCCTGTTTTAACTAAGCT, TAGCTTAGTTAAAACAGGAT, TTAATTTCTGAAGTTGTCCT and CAATTACAACCCTAACCCCT, which resulted in a 274 bp deletion beginning at Chromosome 4 positive strand position 46,144,660 bp GATTGGTTAAGAACTACTCT, and ending after TTTGATTAATTTCTGAAGTTG at 46,144,933 bp (GRCm38/mm10). This mutation deletes exon 2 and 185 bp of flanking intronic sequence including the splice acceptor and donor, and is predicted to cause a change of amino acid sequence after residue 11 and early truncation 18 amino acids later. |
