| Primary Identifier | MGI:5790795 | Allele Type | Targeted |
| Attribute String | Epitope tag, Humanized sequence | Gene | Mecp2 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting construct was designed to insert a loxP-flanked neomycin (neo) resistance cassette upstream of exon 4 of the gene. A missense mutation T158M found in humans carrying the neurological disorder Rett Syndrome (RTT) was introduced to exon 4. A 23 amino acid Tavi tag, allowing MECP2 protein to be biotinylated in the presence of E. coli biotin ligase, BirA, was introduced immediately upstream of Mecp2 stop codon. Cre-mediated recombination removed the upstream floxed neo cassette. |
