| Primary Identifier | MGI:5792831 | Allele Type | Targeted |
| Attribute String | Modified isoform(s), Reporter | Gene | Rhbdf1 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 32162365 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 32165412. The critical exons 4-11 are thus flanked by loxP sites. Cre-mediated recombination removed the neomycin selection cassette and exons 4-11. Quantitative PCR analysis revealed that whereas expression of exons 1-2 is detectable in homozygous mutant mouse embryonic fibroblasts (MEFs), exons 4-5, 12-13, and 16-17 are not expressed. Thus, any protein fragment, if produced in mutant mice, is expected to lack all transmembrane domains and most of the N-terminal cytoplasmic domain. |
