Primary Identifier | MGI:5800310 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Shank3 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene cassette and a second loxP site were inserted into intron 9. Subsquently, an FRT site flanked puromycin resistance gene cassette and a third loxP site were inserted downstream of exon 22. Subsequent flp-mediated recombination removed the two selection cassettes, leaving exons 4 to 9 and 10 to 22 floxed. |