Primary Identifier | MGI:5816528 | Allele Type | Transgenic |
Attribute String | Recombinase, Reporter | Gene | Tg(Npas1-icre,-tdTomato)1Cschn |
Strain of Origin | C57BL/6J | Is Recombinase | true |
Is Wild Type | false |
molecularNote | A mouse bacterial artificial chromosome (BAC) containing the entire Npas1 gene, was modified by inserting iCre-2A (cleavable fusion peptide sequence)-tdTomato and polyadenylation sequence from bovine growth hormone downstream of the ATG translation initiation codon, and removing Npas1 coding sequence. A transgenic insert made from this modified BAC was microinjected into the pronucleus of C57BL/6 fertilized eggs. The resulting Npas1-tdTom transgenic mice from founder line 1 were subsequently maintained on the C57BL/6J background. |