| Primary Identifier | MGI:5817223 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Fars2 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele from project Fars2-8206J-F5419 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences TCCCAGAGTCCCTCTACCCT, CTCTGAGCTACCCAGGGTAG, CATTGTGTAACAGATCACTG and CTAAGAAATAGGGAACGGGG, which resulted in a 467 bp deletion beginning at Chromosome 13 positive strand position 36,231,888 bp CCTGGGTAGCTCAGAGCTTG, and ending after AACATTGTGTAACAGATCAC at 36,232,354 bp (GRCm38/mm10). This mutation deletes exon 3 and 307 bp of flanking intronic sequence including the splice acceptor and donor, and is predicted to cause a change of amino acid sequence after residue 204 and early truncation 35 amino acids later. |
