| First Author | McWilliams TG | Year | 2016 |
| Journal | J Cell Biol | Volume | 214 |
| Issue | 3 | Pages | 333-45 |
| PubMed ID | 27458135 | Mgi Jnum | J:237975 |
| Mgi Id | MGI:5817638 | Doi | 10.1083/jcb.201603039 |
| Citation | McWilliams TG, et al. (2016) mito-QC illuminates mitophagy and mitochondrial architecture in vivo. J Cell Biol 214(3):333-45 |
| abstractText | Autophagic turnover of mitochondria, termed mitophagy, is proposed to be an essential quality-control (QC) mechanism of pathophysiological relevance in mammals. However, if and how mitophagy proceeds within specific cellular subtypes in vivo remains unclear, largely because of a lack of tractable tools and models. To address this, we have developed "mito-QC," a transgenic mouse with a pH-sensitive fluorescent mitochondrial signal. This allows the assessment of mitophagy and mitochondrial architecture in vivo. Using confocal microscopy, we demonstrate that mito-QC is compatible with classical and contemporary techniques in histochemistry and allows unambiguous in vivo detection of mitophagy and mitochondrial morphology at single-cell resolution within multiple organ systems. Strikingly, our model uncovers highly enriched and differential zones of mitophagy in the developing heart and within specific cells of the adult kidney. mito-QC is an experimentally advantageous tool of broad relevance to cell biology researchers within both discovery-based and translational research communities. |
