| Primary Identifier | MGI:5818030 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gpr88 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 2 was flanked by loxP sites and an FRT site flanked neomycin resistance gene cassette with an additional loxP site was inserted downstream in intron 2. Flp-mediated recombination removed the neo cassette. Exon 2 was deleted through Cre-mediated recombination, creating a knock-out allele. In situ hybridization analysis confirmed the absence of transcript in homozygous mutant brains. Furthermore, GPR88-mediated [35S]-GTPgammaS binding was abolished in the striatum, demonstrating lack of GPR88 receptor signaling in mutant mice. |
