Primary Identifier | MGI:5818526 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Kit |
Transmission | Germline | Strain of Origin | 129 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A LoxP-nlacZ-4XPolyA-LoxP-H2B-GFP-FRT-Neo-FRT cassette was targeted to the start codon of kit locus with disruption of endogenous ATG by homologous recombination in ES cells. Flp-mediated recombination removed the neo selection cassette, leaving a single FRT site behind. Resulting mouse line was crossed with Cre expressing mice to remove nlacZ and 4xPloyA stop cassette. |