| Primary Identifier | MGI:5818977 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence, Hypomorph | Gene | Sf3b1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector contains (from 5' to 3') a mutant loxP site (upstream of exon 15), and downstream of exon 16 - a FRT-flanked neomycin/lacZ cassette, a loxP site, inverted copies of exon 15 with the K700E mutation and exon 16, a second mutant loxP site (in opposite orientation to the first mutant loxP site) and a loxP site (in opposite orientation to the first loxP site). Flp-mediated recombination removed the FRT-flanked neo cassette. The K700E (lysine to glutamic acid) substitution is associated with myelodysplastic syndromes. The unrecombined conditional allele is hypomorphic. |
