| Primary Identifier | MGI:5822840 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tnik |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Through homologous recombination a loxP site was inserted into intron 6 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 7. Subsequent cre-mediated recombination deleted out-of-frame exon 7 (which encodes part of the kinase domain), thus creating a null allele with premature stop codons in exon 8. PCR confirmed the absence of exon 7 in transcripts from this allele. |
