| Primary Identifier | MGI:5823262 | Allele Type | Targeted |
| Attribute String | Modified isoform(s) | Gene | Ctbp2 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac x C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The endogenous N-terminal Ribeye A-domain was replaced with a loxP site followed by the Ribeye A domain cDNA fused to GCaMP3 (a GFP based calcium sensor). The coding region is followed by a polyA signal, an FRT-flanked PGK-neomycin selection cassette, and a 3' prime loxP site. The neo cassette is subsequently removed through flp-mediated recombination. The floxed tagged A-domain was deleted through cre-mediated recombination. This removes the possibility of transcribing the Ribeye isoform but leaves the other isoforms intact. |
