| Primary Identifier | MGI:5824213 | Allele Type | Targeted |
| Attribute String | Hypomorph | Gene | Sp7 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector contains a loxP-flanked PGKneo cassette upstream of Biotin-3xFLAG (BioFL) sequence (encoding a biotin recognition motif and 3 copies of the FLAG epitope) inserted at the C-terminus of the Sp7 gene. Cre-mediated recombination removed the upstream floxed neo cassette. Western blotting detects the modified allele at slightly reduced levels as compared to wild-type. Temporal and spatial distributions is equivalent to the endogenous Sp7 gene expression. |
