| Primary Identifier | MGI:5824902 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Zfy2 |
| Strain of Origin | B6D2F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Exon 4 was targed for modification using the CRISPR/Cas9 system. The sgRNA target sequence was 5'-GAAGCAGTCTTAGATTCCAGtgg-3' (PAM sequence in lower case). As this gRNA targets identical sequence in this gene and in Zfy1, the procedure yielded single knock-outs (one founder for each gene) as well as double knock-outs (10 founders). Genotyping of this knock-out allele found in double knock-out founder #2 revealed 6 and 4 bp insertions (2 bp apart) in the target sequence. |
