Primary Identifier | MGI:5825295 | Allele Type | Targeted |
Gene | Scn5a | Transmission | Germline |
Strain of Origin | C57BL/6 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Exon 13 was modified such that an S571A muatation was created in the translated peptide. LoxP sites were inserted in intron 9 and intron 13. An FRT site flanked neomycin resistance gene cassette was inserted between exon 13 and the loxP site. The neo cassette was removed through flp-mediated recombination. The substituted alanine can not be phosphorylated (whereas the original serine can). |