| Primary Identifier | MGI:5828771 | Allele Type | Endonuclease-mediated |
| Attribute String | Hypomorph | Gene | Bcl11a |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The dominant +62 DNase I Hypersensitive Site in a 10kb enhancer region in intron 2 was targeted for deletion with the CRISPR/Cas9 system. In human, mutations in the orthologous enhancer region are associated with various hemoglobin disorders. The gRNA sequences used were 5'-TCGCTGCCTTCAGTTCTGCT-3' and 5'-CTATTTCCATTGGTGGATAC-3'. |
