| First Author | Charles River | Year | 2017 |
| Journal | Scientific info. sheet (http://www.criver.com/files/pdfs/rms/ncg/ncg-mouse-scientific--sheet.aspx) | Mgi Jnum | J:239221 |
| Mgi Id | MGI:5827581 | Citation | Charles River (2017) NOD CRISPR Prkdcil2r Gamma (NCG) Triple-Immunodeficient Mouse Model. Scientific info. sheet (http://www.criver.com/files/pdfs/rms/ncg/ncg-mouse-scientific--sheet.aspx) |
| abstractText | As researchers look for ways to optimize and translate studies from animals to humans, a push to humanize current models has occurred. This has been facilitated by the use of immunodeficient models that permit transplantation of foreign tissues with an attenuated host-versus-graft response. In light of this trend, Charles River Laboratories has acquired a mouse model that displays demonstrable improvements of foreign tissue transplantation and engraftment compared to previous generations of immunocompromised mice. Uniquely created using CRISPR-Cas9 technology to alter the Prkdc and Il2rg genes, this model is triple-immunodeficient, more immunocompromised than commonly used immunodeficient mouse strains, including SCID and nude mice. Prkdc and Il2rg are part of the SCID (severe combined immunodeficiency) family of genes affecting the maturation and formation of T cells, B cells, NK cells, and, to a lesser degree, dendritic cells. Disrupting the Prkdc gene, encoding for the catalytic subunit of the DNA-dependent protein kinase enzyme, reduces function required for V(D)J recombination necessary in propagating antibody diversity of maturing T and B cells. Disrupting Il2rg, encoding for the common gamma subunit found in IL-2 and multiple IL receptors (IL-4, IL-7, IL-9, IL-15, and IL-21), prevents immature lymphocytes (T, B, and NK cells) and other leukocytes from reaching maturation as the receptors are required to bind and induce cytokine-mediated signaling. The NCG mouse strain is similar to other triple-immunodeficient models capable of hosting xenograft cells, tissue and human immune system components, thus enabling studies of tumor biology and immuno-oncology, infectious disease, graft-versus-host disease (GvHD), hematopoiesis, and tissue transplant studies. |
