| Primary Identifier | MGI:5828593 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Ryr2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT site flanked neomycin resistance gene cassette was inserted into intron 31. A green fluorescent protein (GFP) gene was inserted into exon 31, in-frame after the Thr1366 codon in Divergent Region II. To minimise steric hindrance, the GFP gene was flanked both sides by 10-codon linkers rich in glycine codons. The neo cassette was subsequently removed through flp-mediated recombination. |
