| First Author | Ikeda Y | Year | 2013 |
| Journal | FEBS J | Volume | 280 |
| Issue | 22 | Pages | 5884-95 |
| PubMed ID | 24034627 | Mgi Jnum | J:216712 |
| Mgi Id | MGI:5609242 | Doi | 10.1111/febs.12514 |
| Citation | Ikeda Y, et al. (2013) Resistin affects lipid metabolism during adipocyte maturation of 3T3-L1 cells. FEBS J 280(22):5884-95 |
| abstractText | Resistin, an adipose-tissue-specific secretory factor, aggravates metabolic syndrome through impairment of glucose metabolism. Previously, we demonstrated that resistin expression was induced in both 3T3-L1 cells and primary pre-adipocytes derived from Zucker obese rats during the process of differentiation and maturation (Ikeda Y, Hama S, Kajimoto K, Okuno T, Tsuchiya H & Kogure K (2011) Biol Pharm Bull 34, 865-870). However, the biological function of resistin in adipocytes is poorly understood. In the present study, we examined the effects of resistin knockdown on the biological features of 3T3-L1 cells. We found that lipid content was significantly decreased in 3T3-L1 cells transfected with anti-resistin small interfering RNA (siRNA) after adipocyte differentiation. While expression of peroxisome proliferator activated receptor gamma and CCAAT/enhancer-binding protein alpha was not affected, protein expression and transcriptional activity levels of carbohydrate response element binding protein (ChREBP), which upregulates transcription of lipogenic genes, decreased after anti-resistin siRNA treatment. Moreover, gene expression of fatty acid synthase and acetyl-CoA carboxylase 2, which are known to be regulated by ChREBP, were also suppressed by resistin knockdown. In contrast, activity of the fatty acid beta-oxidation-regulating protein carnitine palmitoyltransferase 1 increased. These results suggest that resistin knockdown induces suppression of lipid production and activation of fatty acid beta-oxidation. Consequently, resistin may affect lipid metabolism during adipocyte maturation. |
