| Primary Identifier | MGI:5882077 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Gsdmd |
| Strain of Origin | C57BL/6N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Cytoplasmic injection of Cas9 mRNA and sgRNAs (5'-GAGAAGGGAAAATTTCTGG-3' and 5'-AGGGCAGAGTGATGTTGTC-3') into zygotes resulted in compound heterozygous F1 mice (from F0 founder crosses) and homozygous F2 mice (from F1 crosses). Mutations varied from 1-5 bp insertions to 1-1632 bp deletions. A total of 12 different alleles were generated. |
