| Primary Identifier | MGI:5882386 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Asic3 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 1. mRNA expression levels were studied through RT-PCR and determined that the neo cassette interfered with the transcription of the allele. Therefore the neo cassette was removed through subsequent flp-mediated recombination. Exon 1 was deleted by subsequent cre-mediated recombination, creating a null allele. RT-PCR confirmed depletion of transcripts from this allele. |
