| Primary Identifier | MGI:5897324 | Allele Type | Transgenic |
| Attribute String | Inducible, Inserted expressed sequence | Gene | Tg(Myh6/tetO-Sypl2)1Jmol |
| Strain of Origin | FVB | Induced With | doxycycline/tetracycline |
| Is Recombinase | false | Is Wild Type | false |
| description | Progeny of mice crossed with a Myh6-tTA strain are not viable unless doxycycline (DOX) is administered. Double transgenic animals not treated with DOX develop severe dilated cardiomyopathy and die between 4-8 weeks of age. In the presence of DOX, a greater than 50% loss of SYPL2 expression is seen in the heart. This lower expression of SYPL2 protein in the DOX-treated animals localizes to t-tubules adjacent to RYR2 (ryanodine receptor 2, cardiac) in the apposing SR. |
| molecularNote | A cDNA encoding mouse Sypl2 (synaptophysin like 2; also called mitsugumin 29 or Mg29) is expressed under the regulatory control of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter and the tetracycline responsive element (tetO). |
