| Primary Identifier | MGI:5897147 | Allele Type | Targeted |
| Gene | Fan1 | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Exon 13 of the gene was replaced with a mutated exon where the codon for Lys975 was mutated to encode an alanine. An FRT-flanked puromycin resistance cassette was inserted adjacent to the mutation. Flp-mediated recombination removed the puromycin cassette and left a single FRT site between exon 13 and 14. This allele produces a truncated protein lacking the last 41 amino acids that is devoid of nuclease activity. |
