| Primary Identifier | MGI:5901842 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Polr1c |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele from project Polr1c-8545J-8326M was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences TTGTACATCTGCCTTCGCCT, CCTACTGCTGGGGCTTTAGT, ACCACCCTACCTGCCCCAGA and GGTTCACACCTATGACAGTC, which resulted in a 535 bp deletion beginning at Chromosome 17 negative strand position 46,246,036 bp, CTGGGGCAGGTAGGGTGGTT, and ending after TCTTGTACATCTGCCTTCGC at 46,245,502 bp (GRCm38/mm10). This mutation deletes exon 4 and 402 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 83 and early truncation 7 amino acids later. |
