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Allele : Pdgfrb<tm1.1(cre/ERT2)Csln> platelet derived growth factor receptor, beta polypeptide; targeted mutation 1.1, Chyuan-Sheng Lin
Primary Identifier  MGI:5901894 Allele Type  Targeted
Attribute String  Inducible, Recombinase Gene  Pdgfrb
Transmission  Germline Strain of Origin  (C57BL/6J x 129S6/SvEvTac)F1
Induced With  tamoxifen Is Recombinase  true
Is Wild Type  false
molecularNote  A targeting vector was designed to replace the translation stop codon of the platelet derived growth factor receptor, beta polypeptide (Pdgfrb) gene with a creER fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain) followed by a frt-flanked neomycin resistance (neo) cassette. A viral P2A oligopeptide, from porcine teschovirus-1, was inserted upstream of the creER fusion gene to ribosomal skipping. The construct was electroporated into (C57BL/6J x 129S6/SvEvTac)F1-derived KV1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL6/N blastocysts and resulting chimeric males were bred to Tg(ACTFLPe)9205Dym/J females to remove the neo cassette. These mice express Cre-ER protein in pericytes in the vasculature of organs including brain and retina. Restricted to the cytoplasm, Cre-ER can only gain access to the nuclear compartment after exposure to tamoxifen.
  • mutations:
  • Insertion
  • synonyms:
  • PDGFRbata-P2A-CreERT2,
  • PDGFRbata-P2A-CreERT2
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