Primary Identifier | MGI:5903015 | Allele Type | Targeted |
Attribute String | No functional change, Reporter | Gene | Fgfr1 |
Transmission | Germline | Strain of Origin | 129S4/SvJaeSor |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector is designed to replace the stop codon in exon 18 of the Fgfr1 gene with a cassette containing a human HIST1H2BB gene [histone 1 H2bb] sequence, a viral 2A oligopeptide (T2A) that mediates ribosomal skipping, a cerulean variant of green fluorescent protein (GFP) sequence, and a new stop codon. An FRT-flanked neomycin resistance (neo) cassette was inserted downstream of the new stop codon. Flp-mediated recombination removed the FRT-flanked neo cassette. |