Primary Identifier | MGI:5911623 | Allele Type | Targeted |
Attribute String | Recombinase | Gene | Kiss1 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | true | Is Wild Type | false |
molecularNote | An Frt-neo-Frt-IRES-cre cassette was inserted between the stop codon and polyA site of the Kiss1 gene by homologous recombination in R1 ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then bred to a line expressing Flp recombinase to remove the Frt-flanked neo cassette. |