| Primary Identifier | MGI:7860841 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Tg(Rr27-HBB)del-9Keij |
| Strain of Origin | CD-1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgene insert was generated as follows: two differently modified mouse H19 ICR sequences (del-8 and del-9, each flanked by loxP5171 and loxP2272 sites) were inserted in reverse orientation in human HBB cluster-containing YAC A201F4.3 immediately downstream of the LCR and upstream of the HBB cluster. Subsequent Cre-mediated recombination created separate single del-8 and del-9 transgene alleles. The modifications involves the deletion of the 5' end of the ICR (del-7) and all but the last 420 bp (del-6) of a 478 bp sequence close to the 5' end that confers post-fertilization paternal methylation to the ICR. |
