| Primary Identifier | MGI:7790791 | Allele Type | Endonuclease-mediated |
| Attribute String | Modified regulatory region | Gene | Rr56163 |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The Cyp24a1 proximal promoter region was targeted using sgRNAs (equivalent to GGGGACCGACTGGCAAGGATGGG and CGCTTGCACAATCGCCACTCAGG) and an ssODN template with CRISPR/Cas9 technology, resulting in the mutation of an AP1-binding site from GAGTCA to tttTgt, vitamin-D-response-element 1 (VDRE1) from AGGTGAGTGAGGGCG to ttcTGccTGtttGCG and VDRE2 from GGTTCAGCGGGTGCG to aaTTtAGCtaaccCG. |
