| First Author | Shafaq-Zadah M | Year | 2016 |
| Journal | Nat Cell Biol | Volume | 18 |
| Issue | 1 | Pages | 54-64 |
| PubMed ID | 26641717 | Mgi Jnum | J:230729 |
| Mgi Id | MGI:5763686 | Doi | 10.1038/ncb3287 |
| Citation | Shafaq-Zadah M, et al. (2016) Persistent cell migration and adhesion rely on retrograde transport of beta(1) integrin. Nat Cell Biol 18(1):54-64 |
| abstractText | Integrins have key functions in cell adhesion and migration. How integrins are dynamically relocalized to the leading edge in highly polarized migratory cells has remained unexplored. Here, we demonstrate that beta1 integrin (known as PAT-3 in Caenorhabditis elegans), but not beta3, is transported from the plasma membrane to the trans-Golgi network, to be resecreted in a polarized manner. This retrograde trafficking is restricted to the non-ligand-bound conformation of beta1 integrin. Retrograde trafficking inhibition abrogates several beta1-integrin-specific functions such as cell adhesion in early embryonic development of mice, and persistent cell migration in the developing posterior gonad arm of C. elegans. Our results establish a paradigm according to which retrograde trafficking, and not endosomal recycling, is the key driver for beta1 integrin function in highly polarized cells. These data more generally suggest that the retrograde route is used to relocalize plasma membrane machinery from previous sites of function to the leading edge of migratory cells. |
