| Primary Identifier | MGI:6121439 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gtsf2 |
| Transmission | Germline | Strain of Origin | (C57BL/6J x 129S6/SvEvTac)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site and FRT-flanked pgk-puro cassette were inserted upstream of exon 2 and a loxP site was inserted downstream of exon 4 via homologous recombination. Cre-mediated recombination removed the pgk-puro cassette and exons 2-4. RT-PCR and western blot analyses confirmed the absence of expression in testes from homozygous mice at 8 weeks of age. |
