Primary Identifier | MGI:5910136 | Allele Type | Targeted |
Attribute String | Inducible, Recombinase | Gene | Foxc2 |
Transmission | Germline | Strain of Origin | Not Specified |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
molecularNote | A CreERT2 cassette was inserted downstream of the initiation codon of Foxc2 gene, followed by an Frtflanked PGK-neo sequence via homologouse recombination in embryonic stem (ES) cells. Correctly targeted ES cells were injected into host blastocysts to produce germline chimeric males. Chimeric mice were crossed with Act-FLPe mice to remove the PGK-neo selection cassette. |