| Primary Identifier | MGI:5906262 | Allele Type | Targeted |
| Attribute String | Null/knockout, Recombinase, Reporter | Gene | Il22 |
| Transmission | Germline | Strain of Origin | C57BL/6J |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | Rabbit beta-globin (partial exon 2-3 genomic sequence serving as an artificial intron and splicing donor/acceptor), tandem dimer red fluorescent protein (tdTomato), encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES), huCre (P1 phage cre-recombinase cDNA optimized for human/mammalian cells), bovine growth hormone (bGH) poly-adenylation signal (pA), and a loxP-flanked neomycin cassette were introduced to exon 1 of the mouse Il22 gene via homologous recombination in embryonic stem (ES) cells. The floxed neomycin cassette was excised by through crosses with CMV-cre mice. Mice express tdTomato fluorescent reporter and cre in cells where Il22 is expressed. |
