| Primary Identifier | MGI:5906615 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence, Null/knockout | Gene | Idh2 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S/SvEv)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A c.419G>A mutation was engineered in exon 4, changing arginine codon 140 into a glutamine codon (p.Arg140Gln). This mutation is found in tumors from human accute myeloid leukemia (AML) patients. A loxP site flanked neomycin resistance gene and stop cassette was inserted into intron 3. This creates a conditional-ready null allele that expresses the mutated protein only after cre-mediated recombination. |
