| Primary Identifier | MGI:5906825 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Shh |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site flanked stop and neomycin resistance gene cassette was inserted into the 5' UTR and a mutation was engineered in exon 2. The mutation changes glutamic acid codon 177 (GAA) to an alanine codon (GCG)(p.Glu177Ala). The neo and stop cassette was removed through subsequent cre-mediated recombination. PCR and cDNA sequencing confirm the expression of the mutated transcript from this allele. |
