| Primary Identifier | MGI:5919937 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Rb1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site flanked neomycin resistance gene cassette and the 5.2 kb human pseudogene were inserted into intron 2. The neo cassette was removed through subsequent cre-mediated recombination. The pseudogene contains a CpG island that is differentially (maternally) methylated and has promoter function in human. Mice derived from ES cell clone 42 were used for further breeding. |
