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Allele : Gt(ROSA)26Sor<em1(CAG-cas9*,-EGFP)Dvs> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, David V Serreze
Primary Identifier  MGI:6111351 Allele Type  Endonuclease-mediated
Attribute String  Conditional ready, Endonuclease, Reporter Gene  Gt(ROSA)26Sor
Strain of Origin  NOD/ShiLtJ Is Recombinase  false
Is Wild Type  false
molecularNote  Sequences include CMV enhancer/chicken beta-actin promoter (CAG), a loxP-STOP(3xSV40 polyA)-loxP cassette (LSL), a 3xFLAG sequence, a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]), a P2A ribosomal skip cleavage peptide sequence, an enhanced green fluorescent protein gene (EGFP), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) and a bovine growth hormone polyA sequence (BGHpA). To facilitate insertion into the Gt(ROSA)26Sor locus on chromosome 6 via homology directed repair (HDR), the LSL-Cas9-P2A-EGFP sequences were co-injected with a large piece of Gt(ROSA)26Sor locus homologous DNA.
  • mutations:
  • Insertion
  • synonyms:
  • Rosa26<CAG-LSL-Cas9-P2A-EGFP>,
  • Rosa26<CAG-LSL-Cas9-P2A-EGFP>
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Trail: Allele

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0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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Trail: Allele




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