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Allele : Col1a1<tm1.1(tetO-mStrawberry/Atg4b*C74A)Kmln> collagen, type I, alpha 1; targeted mutation 1.1, Bjorn Knollmann
Primary Identifier  MGI:6111715 Allele Type  Targeted
Attribute String  Inducible, Inserted expressed sequence, Reporter Gene  Col1a1
Transmission  Germline Strain of Origin  C57BL/6NTac
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
molecularNote  The construct contains an mStrawberry red fluorescence protein fused to a modified Atg4b gene under control of a tet-responsive element (TRE; tetO). In this construct, Atg4b contains a dominant negative C74A mutation, which results in the expression of an enzymatically inactive protein. This mStrawberry/Atg4B cDNA fragment was co-electroporated into C57BL/6N-derived C2 embryonic stem (ES) cells along with a pCol-TGM targeting vector, containing an FRT-flanked PGK-Neo inserted into the 3' UTR of the Col1a1 locus, and a pCAGGS-FlpE expression plasmid.
  • mutations:
  • Insertion,
  • Nucleotide substitutions
  • synonyms:
  • ATG4B<C74A>,
  • ATG4B<CA>,
  • ATG4B<C74A>,
  • ATG4B<CA>
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0 Mutation Involves

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