| Primary Identifier | MGI:6113393 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Hes7 |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A lox71 site, a neomycin resistance gene cassette and a lox2272 site were inserted into the 3' UTR in exon 4 immediately after the stop codon. Successfully targeted ES cells were transfected with a cre-expressing vector and a vector containing part of intron 74 of the human dystrophin (DMD) gene. This vector was constructed as follows: a lox66 site, genomic sequence from the last 11 bp of exon 74 up to the first 10 bp of exon 75, and a lox2272 site. The intronic sequence was shortened to 10 kb by deleting sequence from the central portion. Cre-mediated recombination recombined the genomic lox71 site with the vector lox66 site and the genomic and vector lox2272 sites with each other. This results in a knock-in allele with part of the human intron and parts of its flanking exons inserted into the 3' UTR intron of the host gene. RT-PCR, 3'-RACE and in situ hybridization experiments showed that the exogenous intron is not spliced out. |
