Primary Identifier | MGI:6108866 | Allele Type | Targeted |
Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
Is Recombinase | false | Is Wild Type | false |
description | ES cell line = G456, a derivative of ES cell line G4. |
molecularNote | The targeting vector is designed with (from 5' to 3') a ubiquitously expressed CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), loxP-flanked stop cassette, murine lymphoid enhancer binding factor 1 (Lef1) cDNA, a membrane bound T2-Green Fluorescent Protein (T2-mGFP), and a neomycin resistance (neo) cassette inserted into the Gt(ROSA)26Sor locus. |