| First Author | Chang B | Year | 2024 |
| Journal | MGI Direct Data Submission | Mgi Jnum | J:353326 |
| Mgi Id | MGI:7712943 | Citation | Chang B (2024) The D2.Cg-Crb1em2Boc/Boc Strain. MGI Direct Data Submission |
| abstractText | B6.Cg-Crb1<em2Boc> Jak3<m1J>/Boc (JAX Stock No. 031592), which is homozygous for both Crb1<em2Boc> and Jak3<m1J>, has early-onset multifocal depigmented retinal lesions (Figure 1A, 1B). The Crb1<em2Boc> mutation, which was generated in a strain homozygous for both Crb1<rd8> and Jak3<m1J>, is a 17 base pair intragenic deletion in exon 9 from nucleotide 3481-3497 that causes a frameshift with 7 amino acid substitutions before early termination. This double homozygous strain has the same essential phenotype as that of the parental strain, B6.Cg-Crb1<rd8> Jak3<m1J>/Boc (Jax Stock No. 005558), which is referred to as retinal vascularization 3 (rnv3) and includes retinal vascularization with early-onset multifocal depigmented retinal lesions. We have made a new congenic Crb1<em2Boc> strain D2.Cg-Crb1<em2Boc>/Boc (Jax Stock No. 036994) by continuously backcrossing the Crb1<em2Boc> allele from B6.Cg-Crb1<em2Boc> Jak3<m1J>/Boc (JAX Stock No. 031592) onto DBA/2J (Jax Stock No. 000671) for 5 generations. Genotyping confirmed the heterozygous Crb1<em2Boc>/+ F1 mice were used to mate to DBA/2J for each backcross generation. The heterozygous Crb1<em2Boc>/+ N5F1 siblings were mated together to produce N5F2 mice. All N5F2 mice had a normal retina (homozygous Crb1<em2Boc>/Crb1<em2Boc>, heterozygous Crb1<em2Boc>/+ and wild type +/+). The homozygous Crb1<em2Boc>/Crb1<em2Boc> N5F2 siblings were intercrossed to generate a homozygous strain. Mice homozygous for the Crb1<em2Boc> mutation on the DBA/2J genetic background show a normal retina at 4 weeks of age (Figure 1C and 1D) and at 6 months of age (data not show). This strain is useful for genetic analysis identifying the modifier genes in either genetic background, those that enhance the rnv3 phenothype in B6.Cg-Crb1<em2Boc> Jak3<m1J>/Boc or suppress the rnv3 phenotype in D2.Cg-Crb1<em2Boc>/Boc. |
