| Primary Identifier | MGI:6110864 | Allele Type | Targeted |
| Attribute String | Dominant negative, Null/knockout | Gene | Pabpn1 |
| Inheritance Mode | Dominant | Transmission | Germline |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| description | ES cell line = HZ1.1 |
| molecularNote | A loxP site was inserted upstream of exon 1 and an FRT site flanked puromycin resistance gene cassette and a second loxP site into intron 2. The puro cassette was removed through subsequent Flp-mediated recombination. Exons 1 and 2 were deleted through Cre-mediated recombination, creating a null allele. qRT-PCR and immunoblot experiments confirm the lack of expression from this allele. |
