| Primary Identifier | MGI:6140145 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Adamts16 |
| Strain of Origin | C57BL/6NTac | Is Recombinase | false |
| Is Wild Type | false | Project Collection | IMPC |
| molecularNote | This allele from IMPC was generated at Medical Research Council Harwell by injecting CAS9 RNA and 4 guide sequences which resulted in a 906nt deletion. The allele results in a complete loss of exon 5, which encodes part of the highly conserved, zinc-dependent metalloprotease domain; splicing from exon 4 to exon 6 results in a predicted frame-shift and early chain termination due to a premature stop codon. qRT-PCR analysis showed a severe reduction of expression in homozygous mutant kidneys. |
