Primary Identifier | MGI:6159153 | Allele Type | Targeted |
Attribute String | Inserted expressed sequence, Reporter | Gene | Myh9 |
Transmission | Germline | Strain of Origin | 129S/SvEv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A Myh14 cDNA encoding the NMHCII-C1 isoform of mouse non-muscle heavy chain 2C, which includes the 8-amino-acid C1 insertion in loop 1 (from an alternatively spliced exon), fused at its 3' end to the coding sequence for enhanced green fluorescent protein and followed by a loxP-flanked Pgk-neo cassette, was inserted into exon 2 of the NMHCII-A-encoding Myh9 locus immediately downstream of the translation-initiating ATG codon. The selection cassette subsequently was deleted by Cre recombinase. |