| First Author | Hashizume K | Year | 2018 |
| Journal | Genes Brain Behav | Volume | 17 |
| Issue | 2 | Pages | 118-125 |
| PubMed ID | 28782255 | Mgi Jnum | J:259631 |
| Mgi Id | MGI:6149096 | Doi | 10.1111/gbb.12408 |
| Citation | Hashizume K, et al. (2018) POU3F2 participates in cognitive function and adult hippocampal neurogenesis via mammalian-characteristic amino acid repeats. Genes Brain Behav 17(2):118-125 |
| abstractText | POU3F2/BRN-2 is a transcription factor that is mainly expressed in the central nervous system and plays an important role in brain development. The transactivation domain of POU3F2 includes multiple mammalian-characteristic tandem amino acid repeats (homopolymeric amino acid repeats). We previously generated knock-in mice (Pou3f2(Delta/Delta) mice) in which all three homopolymeric amino acid repeats were deleted from the Pou3f2 transactivation domain and identified phenotypic impairments in maternal behavior and pup recognition. Yet, the exact biological implications of homopolymeric repeats are not completely understood. In this study, we investigated cognitive function and hippocampal neurogenesis in Pou3f2(Delta/Delta) mice. Pou3f2(Delta/Delta) mice exhibited cognitive impairment in object recognition and object location tests. Immunohistochemistry for doublecortin, a marker of immature neurons, showed a lower number of newborn neurons in the dentate gyrus of adult Pou3f2(Delta/Delta) mice compared with wild-type mice. Consistent with this observation, adult Pou3f2(Delta/Delta) mice had lower numbers of 5-bromo-2''-deoxyuridine (BrdU) and NeuN double-positive cells at 4 weeks after BrdU injection compared with control mice, indicating the decreased generation of mature granule cells in Pou3f2(Delta/Delta) mice. Taken together, these results suggest that POU3F2 is involved in cognitive function as well as adult hippocampal neurogenesis, and that homopolymeric amino acid repeats in this gene play a functional role. |
