| Primary Identifier | MGI:6152405 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Sp9 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert a FRT site followed by an splice acceptor (SA) fused to the internal ribosomal entry site (IRES) and a lacZ gene, a loxP site, a neomycin resistance gene cassette, a second FRT site and a second loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) intron 1 and a third loxP site immediately downstream of the exon2. Flpe-mediated recombination removed reporter and neo cassette from Sp9tm1Zyan leaving a single FRT site in intoron 1 and loxP flanked exon 2. This allele is a conditional-ready allele. |
