| Primary Identifier | MGI:6154624 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Birc2 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed mini-gene containing exons 4-7 and a BGH poly-A signal were inserted upstream of exon 4, M396A and F398A mutations were inserted in exon 4, and an F3 site flanked pgk-hygro cassette was inserted in upstream of the mutated exon 4 via homologous recombination. Targeting was done in ES cells carrying Birc3tm1Rbr . Flp and cre mediated recombination removed the pgk-hygro cassette and floxed mini-gene. |
