Primary Identifier | MGI:6163634 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Dmd |
Strain of Origin | Not Specified | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 technology generated a 245 bp deletion that completely eliminated exon 50. DNA sequencing of RT-PCR products confirmed deletion of exon 50, which places the gene out of frame. Western blot analysis confirmed absence of protein in skeletal muscle and heart. |